Windows Server 2012 Deployment Proposal Research

Windows Server 2012 Deployment - Research Proposal Example â€Å"Windows Server 2012† is an updated operational tool, which is mainly employed for conducting various tasks with dynamic recovery options. Apparently noted, there lay numerous features of Windows Server 2012 through which WAI can reap significant advantages in the context of getting its internal IT services configured. In this regard, the new facets of Windows Server 2012 can be apparently observed as improved storage, better networking facility, wider remote access, developed server administration, enhanced efficiency, along with fostering innovation and broader flexibility and agility among others (Microsoft, n.d.). It is anticipated that with better execution and incorporation of the aforementioned features, WAI can be able to get its internal IT related services configured with utmost efficacy. Deployment and Server Editions Based on the scenario provided, it can be ascertained that WAI will have two sites particularly in Los Angeles and New York. Since the company will start with 90 employees, the employment of two servers will be sufficient for getting its internal IT services configured. It is worth mentioning that the roles, which will be combined within the requisite two servers, include â€Å"Active Directory Certificate Services†, â€Å"Hyper-V† and â€Å"Active Directory Domain Services† (ADDS) among others. In this similar concern, the role of ADCS will involve in facilitating the servers towards offering certificates to the clients. On the other hand, â€Å"Hyper-V† will play the role of installing as well as configuring the support services within the two servers.

Evocative Object Essay Example | Topics and Well Written Essays - 750 words

Evocative Object - Essay Example ICT refers to technologies that guarantee passage of information through telecommunications. The effects of information and communication technologies reflect on how humans do things in present day life as compared to how they used to in the agrarian economy. The information and communication technologies affect various human fields including health, education, modes of transport, communication, agriculture, governance, security, entertainment, sports, industrialization, and others (Seyler 1). The advancement of information and communication technologies is a continuous process that becomes complex and more effective every day. Nevertheless, these advancements equally have negative effects on the environment where they lead to pollution and security where they promote terrorism. Moreover, they come at a price. However, in most cases the inherent benefits of information and communication technologies are more than the costs accrued. Actually, the benefits of information and communicat ion technologies depend on the mode of application, the gadget itself, government policies, and other external factors. Some of the most common and advanced information and communication technologies include cell phones and computers (Seyler 21). Indeed, there are different versions of computers and cell phones with variant applications that continue to flood the global market. Personally, I have had experiences with technology in many cases in my course of life. However, this paper will illustrate my experience with a cell phone as a form of information and communication technologies. It will equally relate my experience to the society in the context of its technological application. The use of cell phones is growing in the modern world due to the friendly, effective, and advanced applications they contain. Its use varies from different personalities including students, business personalities, doctors, service men, pilots, engineers, and the general society (Seyler 26). As a studen t, I have various uses of my cell phone both inn school and outside school. Indeed, in my very first day in college, I was new in town had a vague idea on where my school is. As such, it was physically impossible to access my school on the registration day. However, iPhone had a GPRS and could thus access Google maps application forming a trusted source of direction. There with just a click on my phones keyboard the goggle maps directed me on the shortest direction from the airport to the school free of charge. This application was so beneficial that my mother actually had to buy an iPhone on her way from work. Again, while at school I have used my cell phone in various significant roles. Indeed, I still remember the day that my friend had an accident along the highway far from town. I used my cell phone again to take pictures of the accident using the video recorder and call the police and the ambulance at the scene of the accident. This saved the life of my friend, reduced traffic snarl at the highway, and ensured timely notification to the authorities. Indeed, I equally used the cell phone to inform my friend’s parents of the accident. Back in school, I use my cell phone in different studies and research. Since my cell phone can access the internet and has a capacity to download and store online information, I frequently use it to further my education. Actually, I use my phone to access emails from my tutors, download assignments, find research materials, and forward my assignments. I do this by connecting my phone to my computer at home using a cable and subsequently use my phone as a modem through tethering. This makes my studying more effective and convenient. In addition, my phone has a calculator that is very relevant in doing calculations at school. Indeed, I use my

The Great Leap Forward launched by Mao in 1958 Essay Example for Free

The Great Leap Forward launched by Mao in 1958 Essay When the Communist party came to power in 1949 after a brutal war against the Nationalists, China was in a devastated state. War against Japan had resulted in the destruction of many of its cities including Beijing. Chinas people were left scared with the horrific memories of the Japanese armys horrendous acts. Rescuing China from the gutter was to prove to be a difficult task for Mao Zedong and his communist comrades. In order to understand the fundamental problems with the Great Leap Forward, previous reforms must be considered in order to fully assess the reasons for the its failures. One of Chinas most notorious problems was land ownership. Most of Chinas land was owned by cruel landlords. Peasants were being exploited and were forced to work long hours for poor pay and terrible living conditions. Mao used this in order to take a fundamental step in assuring that he had total control over the people. He introduced the policy of land reform. Mao re-distributed 40% of Chinas land and gave it to the peasants. This proved to be a truly brilliant political decision as he swept the hearts of the peasants on his side. He realised that as 90% of Chinas population were peasants, he needed to appeal to the masses. This policy was considered a success as an estimated 60% of the entire population benefited form the reform. However, this was to be a sign of Maos disregard for human life as this policy resulted in the death of 2 million landlords, by means of public execution during struggle meetings. Mao also started an early form of collectivisation, by 1952, 40% of peasants were collectivised. The next step was the encouragement of cooperatives, these favoured central management of land under private ownership, and by 1956 80% of peasants were part of cooperatives. The important factor of the land reform policy is that Mao was able to gain support from the peasants, the same peasants he would later use to conduct the Great Leap forward. Despite claiming to be a Marxist, Mao considered rural peasants to be the seeds of agricultural success but thought that industrial peasants were the backbone of the economy. The Great Leap Forward was to be the second economic reform Mao was to launch in China. Inspiring himself from Stalins economic model, in 1953 Mao launched the first 5 year plan. This plan was to be extraordinarily successful. The first of his 5 year plans set high production targets in oil, steel, pig iron and chemical fertiliser. Most of these targets were achieved, notably steel production quadrupled. Mao was able to cut inflation down from 1000% to only 15% by introducing a new currency the Yuan. Maos reforms were all interlinked. He used his social reforms to back up his economic reforms. Mao made revolutionary changes to womens lives in modern China. New sets of laws were introduced giving women the right to work, education and custody rights over their children. This was a significant improvement from the harsh days of foot binding. Mao also deemed it important to educate the Chinese population, another success was his improvement of literacy, and by his death 90% of China was literate. Not only did Mao revolutionise Chinese social life, but he put an end to corruption the government. However, these changes were to contribute to the launch of the great leap forward in a crucial way, by giving women the right to work Mao had significantly enlarged his work force which was important considering his beliefs in mass mobilisation. By the time the he announced the launch of the great leap forward 70% of women were employed. The success of the first 5 year plan can be explained by several factors. The targets set were plausible and most importantly Mao had the help of Russian economic and agricultural experts. However Mao deeply mistrusted experts. Some may argue that this was one of the main reasons for launching the hundred flowers campaign. In order to lure out intellectuals and opposition Mao gave a speech in 1957. During this speech Mao encouraged the intelligentsia to constructively criticize the communist party. At first the movement was slow to take of but once Mao forced the media to get behind it, people started speaking their minds about Maos regime. Communist party members were being heavily criticized and the Chinese people demanded reform. Mao, not uncharacteristically decided to reverse the policy in May 1957. This was to result in a crackdown on the intelligentsia known as the anti rightist campaign. Over 300,000 people were sent to labour camps. The hundred flowers was not simply a way at removing the intelligentsia, it was a way of removing Maos opponents, and this was to make the launch of the Great leap forward less difficult and certainly less questioned for the few experts that remained would be too terrified of speaking against the communist party chairman. The scene is now set for the introduction of the Great Leap Forward. Mao dreamed of transforming China into one of the worlds leading economic powers. Maos dream was to become Chinas nightmare with the launch of the great leap forward in 1958. Maos goal was to transform China into an economic superpower overnight. Many peasants knew little of what the Great Leap forward was for, most thought it was simply a plan to overtake major capitalist countries. However, to serve a higher purpose, Mao saw nuclear power as an essential element to become a superpower. However Maos secrete ambition was expensive. In order to mobilise labour, Mao had to further collectivise cooperatives in the rural parts of China. Mao believed that industry and agriculture were equally important, hence the slogan walking on two legs. However, the first 5 year plan had been beneficial to industry but agriculture had stagnated. One of Maos main concerns was Chinas population was outgrowing food production. In 1957 food production had grown 1% whilst the population had grown by 2%. Mao was distraught by the fact that the countrysides production was being used up in by the rural population. This posed a real economic problem for China. It meant that industry was not going to be sufficiently supported by agriculture and thus meant that Maos ambitions could not be realised. Maos answer to this problem was to decentralise control and enable enlarged agricultural units produce food and industrial products. These new super collectives would be known as Peoples Communes. These communes were under the control of local cadres whos main order were to extract as much labour as possible from the peasants. These cadres forced peasants were forced to hand over their property, thus reversing his policy of Land reform. The first of people communes was created in Henan in April 1958. It was composted of 27 collectives with over 9369 households joined together, by December 1958, 740,000 cooperatives had been turned into 26,000 communes. Mao had successfully militarised Chinas society, militia units squads were formed and were composed of everyone between 15 to 20 years of age. Living conditions in the communes were nothing short of appalling. Peasants eat, slept and washed together. All privacy was swept away from them, Mao even considered getting rid of peoples names and replacing them with numbers. According to Jung Chang and Jon Halliday Mao aim was to dehumanise Chinas 550 million peasants and turn them into the human equivalent of draft animals Mao had betrayed the peasants and was going to trade the peasants life for economic growth. Mao expected far too much from these communes. This may explain why the harvest predictions were astronomically high. Mao would have done well to examine the previous harvesting results. The normal yield was a ton per acre. The previous harvest of 1957 yielded a poor 195 million tons of grain. In 1958 Mao announced that the harvest figures for that year had been 430 million tons, western experts place this figure around 200 million tons. This demonstrates how much the production figures were exaggerated. Mao s political secretary Chen Boda told Mao that China was accomplishing in a day what it took capitalist states 20 years to accomplish. Production actually decreased during the Great Leap Forward by significant amounts, the harvest of 1959 was yielded a disappointing 170 million tons the CCP reported it at 282 million tons. This figure was to get even lower in 1960 when it fell to 143 million tons. This can be attributed to poor agricultural techniques. Close planting and deep ploughing were considered to be at the hear t of agricultural success. During these years Mao was asked how he intended to pay for his newly ordered soviet heavy machinery. Mao answered by claiming that China has unlimited food supplies. Consequently China increased its food exports towards Russia.

The Optical Time Domain Reflectometer Essay Example for Free

The Optical Time Domain Reflectometer Essay In fiber optic networks, OTDR (Optical Time Domain Reflectometer) is an opto-electronic instrument used to characterize an optical fiber. Unlike power meters OTDR does not measure loss, but instead implies it by looking at the backscatter signature of the fiber. Generally, OTDR are used to determine the loss of any part of a system, the length of the fiber and the distance between any points of interest. Most of the light which is sent to the fiber can be detected at the other end, but a part of it is always absorbed or scattered. Absorption and scattering are caused by imperfections of fiber, small grains of dirt, for instance. Scattering means that light is not absorbed but it is just sent in different angle after it hits small particles in optical fiber (Figure 1). Some of the light is scattered to the direction it came from. This is called backscattering. Backscattering forms the basis to the use of the optical time domain reflectometry. Figure 1 Rayleigh –scattering in optical fiber Optical time domain reflectometry is based on scattering and reflections. OTDR sends an optical pulse to the fiber and measures the received backscattering. The signal which is received consists naturally only of scattering and reflections of pulse which was sent. By interpreting signal as a function of time OTDR can draw an attenuation of a fiber as a function of distance. Theory of the OTDR Optical time domain reflectometry measures backscattering as a function of time and graph is then drawn as a function of distance (Figure 2). The graph represents the power of signal which the detector of the OTDR receives. The graph of fiber probed by OTDR consists of two spikes with gradually decreasing line between them. The line between spikes is decreasing because the received signal is decreased as a function of distance in accordance with attenuation coefficient of fiber. At the both ends of fiber reflection is large (Fresnel reflection) which creates spikes to the graph. Length of the fiber can therefore be measured from the width of the graph. Figure 2 OTDR signal as a function of distance An OTDR trace is a graphical representation of optical changes or events on a fiber. An event could be a splice, optical connector, a bend, a break, or just normal backscattered light from the fiber itself. In the OTDR trace faults for instance, are shown as a drop in the power of received signal (Figure 3). Size of a drop depends on an amount of power that is lost due to the component. The lost power represents of course the attenuation of component. Components and faults in fiber are either reflective or nonreflective. Reflective components create a spike to the graph of OTDR the same way as the both ends of fiber do. With nonreflective components there are no spikes because no excess light is reflected back. In most cases reflective attenuation is caused by connectors or other passive components and nonreflective attenuation is usually caused by fusion splice or similar fault in fiber. Figure 3 Attenuation of different faults Figure 4 OTDR Trace Information The slope of the OTDR trace shows the attenuation coefficient of the fiber and is calibrated in dB/km by the OTDR (Figure 4). Whereby, The height of that peak will indicate the amount of reflection at the event, unless it is so large that it saturates the OTDR receiver. Then the peak will have a flat top and tail on the far end, indicating the receiver was overloaded. Sometimes, the loss of a good fusion splice will be too small to be seen by the OTDR. Thats good for the system but can be confusing to the operator. It is very important to know the lengths of all fiber in the network so that the operator is not confused by unusual events. Reflective pulses show the resolution of the OTDR. Two events which are closer than the pulse width cannot be seen. Generally longer pulse widths are used to be able to see farther along the cable plant and narrower pulses are used when high resolution is needed, although it limits the distance the OTDR can see. The Dead Zone Dead zones originate from reflective events (connectors, mechanical splices, etc.) along the link, and they affect the OTDR’s ability to accurately measure attenuation on shorter links and differentiate closely spaced events, such as connectors in patch panels, etc. When the strong optical reflection from such an event reaches the OTDR, its detection circuit becomes saturated for a specific amount of time (converted to distance in the OTDR) until it recovers and can once again measure backscattering accurately. As a result of this saturation, there is a part of the fiber link following the reflective event that can not be â€Å"seen† by the OTDR. Analyzing the dead zone is very important to ensure the whole link is measured. Two types of dead zones are usually specified: 1. Event dead zone: This refers to the minimum distance required for consecutive reflective events to be â€Å"resolved†, i.e., to be differentiated from each other. If a reflective event is within the event dead zone of the preceding event, it will not be detected and measured correctly. Industry standard values range from 0.8 m to 5 m for this specification. Figure 5 Common OTDR with 3 m event dead zone 2. Attenuation dead zone: This refers to the minimum distance required, after a reflective event, for the OTDR to measure a reflective or non-reflective event loss. To measure short links and to characterize or find faults in patchcords and leads, the shortest possible attenuation dead zone is best. Industry standard values range from 3 m to 10 m for this specification. To overcome the problem of dead zones, usually a patchcord of about 100 m is added at the beginning of the system. As a result, all lauch dead zone problems have finished before the fiber (which is to be tested) is reached. Ghosts When testing short cables with highly reflective connectors, it is likely to encounter ghosts like in Figure 6. These are caused by the reflected light from the far end connector reflecting back and forth in the fiber until it is attenuated to the noise level. Ghosts are very confusing, as they seem to be real reflective events like connectors, but will not show any loss. If a reflective event in the trace is found at a point where there is not supposed to be any connection, but the connection from the launch cable to the cable under test is highly reflective, look for ghosts at multiples of the length of the launch cable. Figure 6 OTDR Ghosts Resolution of the OTDR Consider that light travels 1 m every 5 ns in the fiber, so a pulsewidth of 100 ns would extend for a distance of 20 m. When the light reaches an event, such as a connector, the light is reflected. The reflection appears to be a 20 m pulse on the OTDR. However, if two events are separated by a distance of 10 m or less (Figure 7), the two reflections will overlap and join up in returning to the OTDR. Figure 7 Thus the OTDR will display the two events as one event and the loss at each event is not detected, instead the sum of losses at both events will be shown on the OTDR. Choosing a shorter pulsewidth may give a better resolution but in turn resulting a low energy content (causing shorter detection range). Besides using a shorter pulse which will provide the required range, a tool that is called a â€Å"visual fault locator† can help too. The visual fault locator injects a bright red laser light into the fiber to find faults. If there is a high loss, such as a bad splice, connector or tight bend stressing the fiber, the light lost may be visible to the naked eye. This will resolve event which is close to the OTDR or close to another event that are not resolvable to the OTDR. The limitation of this tool is about 4 km.

The Ash Content Of A Crude Drug Biology Essay

The Ash Content Of A Crude Drug Biology Essay The ash content of a crude drug is generally taken to be the residue remaining after incineration. It usually represents the inorganic salts naturally occurring in the drug and adhering to it, but it may also include inorganic matter added for the purpose of adulteration. There is a considerable difference varies within narrow limits in the case of the same individual drug. Hence an ash determination furnishes a basis for judging the identity and cleanliness of a drug and gives information relative to its adulteration with inorganic matter. Ash standards have been established for a number of official drugs. Usually these standards get a maximum limit on the total ash or on the acid insoluble ash permitted. The total ash is the residue remaining after incineration. The acid insoluble ash is the part of the total ash which is insoluble in diluted hydrochloric acid. The ash or residue yielded by an organic chemical compound is as a rule, a measure of the amount of inorganic matters present as impurity. In most cases, the inorganic matter is present in small amounts which are difficult to remove in the purification process and which are not objectionable if only traces are present. Ash values are helpful in determining the quality and purity of the crude drugs in powder form. Procedures given in Indian pharmacopoeia were used to determine the different ash values such as total ash and acid insoluble ash. Total ash Weighed accurately about 3 gm of air dried powdered drug was taken in a tarred silica crucible and incinerated by gradually increasing the temperature to make it dull red until free from carbon cooled and weighted and then calculated the percentage of total ash with reference to the air dried drug. Acid insoluble ash The ash obtained as directed under total ash above was boiled with 25 ml of 2N HCl for 5 minutes. The insoluble matter was collected on ash less filter paper, washed with hot water ignited and weighed, then calculated the percentage of acid insoluble ash with reference to the air dried drug. Water soluble ash The total ash obtained was boiled with 25 ml of water for 5 minutes. The insoluble matter was collected on an ash less filter paper, washed with hot water and ignited for 15 minutes at a temperature not exceeding 450ËÅ ¡C. The weight of insoluble matter was subtracted from the weight of total ash. The difference in weight represents the water soluble ash. The percentage of water soluble ash calculated with reference to the air dried drug. b. EXTRACTIVE VALUES Extractive values of crude drugs are useful for their evaluation, especially when the constituents of a drug cannot be readily estimated by any other means. Further, these values indicate the nature of the constituents present in a crude drug. Determination of alcohol soluble extractive value 5 gm of the air-dried coarse powder of Anogeissus latifolia wall (Roxb.ex.DC) was macerated with 100 ml of 90% ethanol in a closed flask for 24 hours, shaking frequently during the first 6 hours and allowing standing for 18hours. Thereafter, it was filtered rapidly taking precautions against the loss of the solvent. Out of that filtrate, 25 ml of the filtrate was evaporated to dryness in a tarred flat bottomed shallow dish, dried at 105ËÅ ¡C and weighed. The percentage of ethanol soluble extractive value was calculated with reference to the air- dried drug. The results are recorded in the table. Determination of water soluble extractive value Weigh accurately 5 gm of coarsely powdered drug and macerate it with 100 ml of chloroform water in a closed flask for 24 hours, shaking frequently during the first 6 hours and allow to standing for 18 hours. Thereafter, it was filtered rapidly taking precautions against loss of the solvent. Then 25 ml of the filtrate was evaporated to dryness in a tarred flat bottomed shallow dish, dried at 105ËÅ ¡C and weighed. The percentage of water soluble extractive was calculated with reference to the air dried drug. The results are given in the table. c. LOSS ON DRYING Loss on drying is the loss in weight in percentage w/w determined by means of the procedure given below. It determines the amount of volatile matter of any kind (including water) that can be driven off under the condition specified (Desiccators or hot air oven). If the sample is in the form of large crystals, then reduce the size by quick crushing to a powder. Procedure About 1.5 gm of powdered drug was weighed accurately in a tarred porcelain dish which was previously dried at 105ËÅ ¡C in hot air oven to constant weight and then weighed. From the difference in weight, the percentage loss of drying with reference to the air dried substance was calculated. d. FLUORESCENCE ANALYSIS [Kokate.C.K, 2002; Khandelwal KR 1996]. In the near-ultra region of the spectrum (3000-4000AËÅ ¡) some of the phytoconstituents show more or less brilliant coloration when exposed to radiation. This phenomenon of emitting visible wavelengths as a result of being excited by radiation of a different wavelength is known as fluorescence. Sometimes the amount of ultra-violet light normally present with visible light is sufficient to produce the fluorescence, but often a more powerful source of ultra-violet is necessary, e.g. mercury vapour lamp. It is often possible to make use of this phenomenon for the qualitative examination of herbal drugs. A fluorescence characteristic of the powdered leaves of Anogeissus latifolia wall (Roxb.ex.DC) was observed in daylight and UV light. Also the fluorescent study was performed on treating the drug powder with different chemical reagents. The observed results are given in table. e. FOAMING INDEX: [Divakar M.C., 1996] Foaming index is mainly performed to determine the saponin content in an aqueous decoction of plant material. Determination of foaming index: Weighed accurately about 1g of coarsely powdered drug and transformed to 500ml conical flask containing 100ml of boiling water. Maintained at moderate boiling at 80-90ËÅ ¡c for about 30min. Cooled and added sufficient water through the filter to make up the volume to 100ml (V1). Cleaned 10 stoppered test tube of uniform dimension were taken and transferred the successive portions of 1,2,3ml up to 10ml and adjusted the volume of the liquid in each test tube with water to 10ml.Stoppered the tubes and shaken them in a lengthwise motion for 15 sec uniformly and allowed to stand for 15min and measure the height of foam. If the height of the foam in every tube is less than 1cm, the foaming index is less than 100(not significant). Here the foam was more than 1cm height after dilution of plant material. If the height of the foam in every tube is more than 1cm, the foaming index is more than 1000. In this case, 10ml of first decoction of plant material is measured and transferred to 100m l volumetric flask (V2) and volume is made to 100ml and followed the same procedure. 5.1. 2. PRELIMINARY PHYTOCHEMICAL ANALYSIS Extraction of plant material:- Petroleum ether extraction:- About 400gm of dry coarse leaf powder of the Anogeissus latifolia wall (Roxb.ex.DC) was extracted with petroleum ether 2500ml (40-600c) for 18 hrs by continuous hot percolation method. It was allowed to cool to 40oC and then filtered using whatman No.1 filter paper. The filtrate was then concentrated in a rotary evaporator and the extract stored at 4 °C until required. The extract yield (% w/w) from the plant material was recorded. Methanolic extraction:- About 400g of air dried coarse powdered material was taken in 1000ml soxhlet apparatus and soaked with petroleum ether for 2 days. At the end of second day the powder was taken out and it was dried. After drying it was again packed and extracted by using methanol (Changshu yangyuan chemicals, China) as solvent, till colour disappeared. The temperature was maintained at 55 °C-65 °C. After that extract was concentrated by distillation and solvent was recovered. The final solution was evaporated to dryness. The colour, consistency and yield (% w/w) of methanolic extract were noted. S.No. Name of extract Colour Consistency Yield% W/W 1 2 Methanolic extract Petroleum ether extract Blackish brown Blackish green Non Sticky mass sticky oily mass 16.75 1.60Table: 1. Nature and colour of extract of Anogeissu latifolia wall (Roxb.ex.DC). 5.1. 3 CHEMICAL TESTS: A) Test for carbohydrates 1. Molisch Test: It consists of treating the compounds of a-naphthol and concentrated sulphuric acid along the sides of the test tube. Purple colour or reddish violet colour was produced at the junction between two liquids. (Kokate, C.K et al, 2000) 2. Fehlings Test: Equal quantity of Fehlings solution A and B is added. Heat gently, brick red precipitate is obtained. 3. Benedicts test: To the 5ml of Benedicts reagent, add 8 drops of solution under examination. Mix well, boiling the mixture vigorously for two minutes and then cool. Red precipitate is obtained. 4. Barfoeds test: To the 5ml of the Barfoeds solution add 0.5ml of solution under examination, heat to boiling, formation of red precipitate of copper oxide is obtained. B) Test for Alkaloids 1. Dragendroffs Test: To the extract, add 1ml of Dragendroffs reagent Orange red precipitate is produced. 2. Wagners test: To the extract add Wagner reagent. Reddish brown precipitate is produced. 3. Mayers Test: To the extract add 1ml or 2ml of Mayers reagent. Dull white precipitate is produced. 4. Hagers Test: To the extract add 3ml of Hagers reagent yellow Precipitate is produced. C) Test for Steroids and Sterols 1. Liebermann Burchard test: Dissolve the test sample in 2ml of chloroform in a dry test tube. Now add 10 drops of acetic anhydride and 2 drops of concentrated sulphuric acid. The solution becomes red, then blue and finally bluish green in colour. 2. Salkowski test: Dissolve the sample of test solution in chloroform and add equal volume of conc. sulphuric acid. Bluish red cherry red and purple color is noted in chloroform layer, whereas acid assumes marked green fluorescence. D) Test for Glycosides 1. Legals test: Sample is dissolved in pyridine; sodium nitropruside solution is added to it and made alkaline. Pink red colour is produced. 2. Baljet test: To the drug sample, sodium picrate solution is added. Yellow to orange colour is produced. 3. Borntrager test: Add a few ml of dilute sulphuric acid to the test solution. Boil, filter and extract the filtrate with ether or chloroform. Then organic layer is separated to which ammonia is added, pink, red or violet colour is produced in organic layer. 4. Killer Killani test: Sample is dissolved in acetic acid containing trace of ferric chloride and transferred to the surface of concentrated sulphuric acid. At the junction of liquid reddish brown color is produced which gradually becomes blue. E) Test for Saponins Foam test: About 1ml of alcoholic sample is diluted separately with distilled water to 20ml, and shaken in graduated cylinder for 15 minutes.1 cm layer of foam indicates the presence of saponins. F) Test for Flavonoids Shinoda test: To the sample, magnesium turnings and then concentrated hydrochloric acid is added. Red colour is produced. G) Test for Tri-terpenoids In the test tube, 2 or 3 granules of tin was added, and dissolved in a 2ml of thionyl chloride solution and test solution is added. Pink colour is produced which indicates the presence of triterpenoids. H) Tests for Tannins and Phenolic Compounds: To 2-3 ml of extract, add few drops of following reagents: a). 5% FeCl3 solution: deep blue-black color. b). Lead acetate solution: white precipitate. c). Gelatin solution: white precipitate d). Bromine water: decolouration of bromine water. e). Acetic acid solution: red color solution f). Dilute iodine solution: transient red color. g). Dilute HNO3: reddish to yellow color. I) Test for Fixed Oils and Fatty acids a). Spot test: Small quantity of the extract is placed between two filter papers. Oil stain produced with any extract shows the presence of fixed oils and fats in the extracts. b). Saponification test: Few drops of 0.5N alcoholic potassium hydroxide are added to the extract with few drops of phenolphthalein solution. Later the mixture is heated on water bath for 1-2 hours soap formation indicates the presence of fixed oils and fats in the extracts. J) Test for Gums and Mucilage: a). Ruthenium red test: Small quantities of extract are diluted with water and added with ruthenium red solution. A pink colour production shows the presence of gums and mucilage. K) Test for Proteins and Amino acids Biuret test: Add 1 ml of 40% sodium hydroxide and 2 drops of 1% copper sulphate to the extract, a violet colour indicates the presence of proteins. Ninhydrin test: Add 2 drops of freshly prepared 0.2% Ninhydrin reagent to the extract and heat. A blue colour develops indicating the presence of proteins, peptides or amino acids. Xanthoprotein test: To the extract, add 20% of sodium hydroxide or ammonia. Orange colour indicates presence of aromatic amino acid. 5.1. 4.TOXICOLOGICAL EVALUATION Determination LD50 value of Anogeissus latifolia (Roxb.ex.DC).wall.Gullperr Acute Oral Toxicity Study The procedure was followed by using OECD guidelines 423 (Acute toxic class method) Animals: Adult albino rats (Wister strain) of either sex with weighing 150 180gm were used. The animals were maintained on the suitable nutritional and environmental condition throughout the experiment. The animals were housed in polypropylene cages with paddy house bedding under standard laboratory condition for an acclimatization periods of 7 days prior to performing the experiment. The animals had access to laboratory chow and water. The experimental protocols were approved by institutional Animal Ethical Committee a written permission from in house ethical committee has been taken to carry out (Reference no. JKKMMRF/2010/009) and complete this study. Procedure: Twelve animals (Wister Albino rats, 150-200gm) were selected for studies. The acute toxic class method is a step wise procedure with 3 animals of single sex per step. Depending on the mortality and / or moribund status of the animals, on average 2-4 steps may be necessary to allow judgment on the acute toxicity of the test animals while allowing for acceptable data based scientific conclusion. The method uses defined doses (5, 50, 300, 2000 mg / kg body weight) and the results allow a substance to be ranked and classified according to the Globally Harmonized system (GHS) for the classification of chemical which cause acute toxicity. Most of the crude extracts possess LD50 value more than 2000 mg. /kg of the body weight of animal used. Dose volume was administered 0.1 ml / 100 gm body weight to the animal by orally after giving the dose the toxic signs were observed within 3-4 hours. Body weight of animals before and after administration, onset of toxicity and signs of toxicity like changes in skin and fur, eyes, and mucous membrane and also respiratory, circulatory, autonomic and central nervous systems and somatomotor activity and behavior pattern, signs of tremors, convulsion, salivation, diarrhoea, lethargy, sleep and coma was also to be noted, if any , was observed. Observation No toxicity or death was observed for these given dose levels, in selected and treated animals. So the LD 50 of the Anogeissus latifolia wall (Roxb.ex.DC), as per OECD guidelines-423 is greater than 2000mg/kg (LD50 > 2000mg/kg). Hence, the biological dose was fixed at 200, 400 and 600mg/kg of body weight for the extract. PHARMACOLOGICAL EVALUATION 5.2.1 Evaluation of Anti-ulcer Activity:- Animals used: Adult albino rats (Wister strain) of either sex with weighing 150 180gm were used. The animals were maintained on the suitable nutritional and environmental condition throughout the experiment. The animals were housed in polypropylene cages with paddy house bedding under standard laboratory condition for an acclimatization periods of 7 days prior to performing the experiment. The animals had access to laboratory chow and water. The experimental protocols were approved by institutional Animal Ethical Committee a written permission from in house ethical committee has been taken to carry out (Reference no. JKKMMRF/2010/009) and complete this study. 5.2.2 Experimental procedure Ethanol induced ulcer:- Male albino-Wistar rats were divided in to five groups of six animals per group and animals were fasted for 24 hrs prior to the experiment in perforated steel cages to avoid coprophagy. Six groups were made as below Group I animals served as normal controls. Group II received 1% CMC (1.0ml/kg p.o) as vehicle control. Group III received 200mg/kg, p.o methanolic extract of Anogeissus latifolia. Group IV received 400mg/kg, p.o methanolic extract of Anogeissus latifolia. Group V received 100mg/kg, Sucralfate as standard One hour after the drug treatment the animals were treated with absolute ethanol [5ml/kg] to induce ulcers. The animals were sacrificed after 1hrs and stomach was opened and percentage inhibition of ulcer was determined. (Mozafar khazaei et al., 2006, Paul V. et al 2002, Paul V. et al., 2000) Aspirin induced ulcer:- Male albino-Wistar rats were divided in to five groups of six animals per group and animals were fasted for 24 hrs prior to the experiment in perforated steel cages to avoid coprophagy. Six groups were made as below Group I animals served as normal controls. Group II received 1% CMC (1.0ml/kg p.o) as vehicle control. Group III received 200mg/kg, p.o methanolic extract of Anogeissus latifolia. Group IV received 400mg/kg, p.o methanolic extract of Anogeissus latifolia. Group V received 100mg/kg, Sucralfate as standard One hour after the drug treatment the animals were treated with aspirin [200 mg/kg] to induce ulcers. The animals were sacrificed after 1hrs and stomach was opened and percentage inhibition of ulcer was determined. (Mozafar khazaei et al., 2006, Paul V. et al 2002, Paul V. et al., 2000) 5.2.3 BIOCHEMICAL PARAMETERS:- The stomach was carefully excised keeping oesophagus closed and opened along greater curvature and luminal contents were removed. The gastric contents were collected in a test tube and centrifuged. The gastric contents were analyzed for gastric juice volume, pH, free and total acidity. 5.2.4 Measurement of gastric juice volume and pH:- Gastric juice was collected from ethanol induced ulcer rats. The gastric juice thus collected was centrifuged at 3000 rpm for 10 min. The volume of supernatant was measured and expressed as ml/100g body weight. The pH of the supernatant was measured using digital pH meter. (Canmon DC. et al., 1969, Kannappan et al., 2008, Patil K.S. et al., 2008, Paul V. et al., 2000) 5.2.5 Determination of free and total acidity:- An aliquot of 1.0 ml of gastric juice was pipette out in to a 50 ml conical flask and 2/3 drops of Topfers reagent was added to it and titrated with 0.01N NaOH until all traces of the red colour disappeared and the colour of the solution turned yellowish orange. The volume of 0.01N NaOH was noted which corresponds to free acidity. Then 2/3 drops of phenolphthalein was added and titration was continued until a permanent pink colour was developed. The volume of total alkali consumed was noted which corresponds to total acidity. The free acidity and total acidity was determined using the formula and values are expressed as mEq/l 100g. (Kannappanetal. 2008, Rajkapoor et al., 2002). Acidity = Volume of NaOH X Normality of NaOH X 100 (mEq/L per 100g) 0.01 5.2.6 Ulcer index (UI):- The mucosa was flushed with saline and stomach was pinned on frog board. The lesion in glandular portion was examined under a 10x magnifying glass and length was measured using a divider and scale and gastric ulcer was scored. Ulcer index of each animal was calculated by adding the values and their mean values were determined. (Malairajan et al., 2007) 0 Normal coloured stomach 0.5 Red colouration 1 Spot ulceration 1.5 Haemorrhagic streak 2 ulcers 3 Perforations 5.2.7 Percentage inhibition: Percentage inhibition was calculated using the following formula. (Malairajan et al., 2007) UI ulcer control UI ulcer treated % inhibition = X 100 UI ulcer control 5.2. 8. Statistical Analysis: All the values are expressed as mean  ± S.E.M for groups of six animals each. Analyzed by one way ANOVA and compared by using Tukey- Kramer multiple comparison tests. The values are statistically significant at three levels, ***p 0.05. 5.3. EVALUATION OF DIURETIC ACTIVITY Animals used: Adult albino rats (Wister strain) of either sex with weighing 150 180gm were used. The animals were maintained on the suitable nutritional and environmental condition throughout the experiment. The animals had access to laboratory chow and water. The experimental protocols were approved by institutional Animal Ethical Committee a written permission from in house ethical committee has been taken to carry out (Reference no. JKKMMRF/2010/009) and complete this study. Experimental procedure The method of (Lipchitz et.al., 1943) was employed for the evaluation of diuretic activity. The Male Albino-Wistar rats were divided into four groups of six rats in each as mentioned below. Group I received Normal saline (25mg/kg, p.o) as control. Group II received (400mg/kg, p.o) methanolic extract of Anogeissus latifolia. Group III- received (600mg/kg, p.o) methanolic extract of Anogeissus latifolia. Group IV received Furosemide (20mg/kg, p.o) as standard. The animals were fasted and deprived of food and water for 18hour prior to the experiment. On the day of experiment, the group I animals serving as control, received normal saline (25ml/kg,p.o), the group II animals received methanolic extract of Anogeissus latifolia wall (Roxb.ex.DC) leaves (400mg/kg,p.o) and group III animals also received methanolic extract (600mg/kg,p.o), the group IV animals received Furosemide (20mg/kg,p.o), respectively, in normal saline. Immediately after the administration the animals were kept in metabolic cages (three per cage) specially designed to separate urine and fecal matter and kept at room temperature of 25  ± 0.5 ° C throughout the experiment. The total volume of urine was collected at the end of 5hrs after dosing. During this period no water and food was made available to the animals. The parameters taken for individual rat were body weight before and after test period, total concentration of Na+ , K+ and Cl ­- in the urine. The Na+ and K+ were measured by flame photometry and Cl ­- concentration was estimated by titration with silver nitrate (N/50) using three drop of 5% potassium chromate solution as indicator .the results are reported as mean  ±SD, the test of significance (P 5.3.1. Statistical analysis: All the values are expressed as mean  ± S.E.M for groups of six animals each. Analyzed by one way ANOVA and compared by using Tukey- Kramer multiple comparison tests. The values are statistically significant at three levels, ***p 0.05. 5.4 EVALUATION OF ANALGESIC ACTIVITY Animals used: Adult albino rats (Wister strain) of either sex with weighing 150 180gm were used. The animals were maintained on the suitable nutritional and environmental condition throughout the experiment. The animals were housed in polypropylene cages with paddy house bedding under standard laboratory condition for an acclimatization periods of 7 days prior to performing the experiment. The animals had access to laboratory chow and water. The experimental protocols were approved by institutional Animal Ethical Committee a written permission from in house ethical committee has been taken to carry out (Reference no. JKKMMRF/2010/009) and complete this study. Procedures: Eddys hot plate method: The Male Albino-Wistar rats were divided into four groups of six rats in each as mentioned below. Group I received 1% CMC (3ml/kg, p.o) as control. Group II received (400mg/kg, p.o) methanolic extract of Anogeissus latifolia. Group III- received (600mg/kg, p.o) methanolic extract of Anogeissus latifolia. Group IV received pentazocine (5mg/kg, p.o) as standard Analgesic activity was performed by using Eddys hot plate (Inco, India) maintained at a temperature of 55 ±1 °c. The basal reaction time of all animals towards thermal heat was recorded. The animals which showed forepaw licking or jumping response within 6-8 seconds were selected for the study. Male Albino rats were divided into 5 groups having 6 animals each and they were divided into 5 groups having 6 animals each and they were fasted overnight during the experiment free access to water. Group first received 1 % CMC (3ml/kg, p.o).Group second, third and fourth received methanolic extract of Anogeissus latifolia (Roxb.ex DC.) wall. Gull perr leaves of dose 400mg/kg and 600mg/kg, orally as a suspension in 1%CMC solution respectively Group five received Pentazocine (5mg/kg, p.o) as reference drug . 60 mins after the administration of test and reference compounds, the animals in all the six groups were individually exposed to the plate maintained at 55 °c and observations were recorded for 3 hours. The time taken in seconds for fore paw licking or jumping was taken as reaction time. A cut off period of 15 seconds is observed to avoid damage to the paws. The percentage protection was calculated using the formula, Percentage protection = (T/C-1) ÃÆ'-100 where, T is the reaction time of treated group and C the reaction time of control group.

Responsibility for the Deaths of Romeo and Juliet in William Shakespear

Taking Responsibility for the Deaths of Romeo and Juliet in William Shakespeare's Romeo and Juliet Romeo and Juliet, the star crossed lovers, the perfect sadly losing there lives for their great love of each other. The tragic death has many characters to blame, but it is impossible to pinpoint the full responsibility on one individual character. Many of the characters in 'Romeo and Juliet' blame each other, for example, the Prince blames the two families and feud for their deaths. He believes that if certain things were different than this whole mess would not have occurred. If this terrible feud were not occurring than there would have been no objection to Romeo and Juliet getting married. "Where be these enemies? Capulet, Montague, see what a scourge is laid apon your hate," ====================================================================== (Act5 Scene3 lines 291-292) (The Prince) The most obvious character to blame in 'Romeo and Juliet' is most probably Friar Lawrence, for it was him who invents the bizarre scheme that will bring them together. Romeo puts his faith and trust in the Friar and he treats him almost like his own father. The Friars character is a very selfish one. At first he shows care and concern for Romeo by not totally agreeing with Romeos actions and decisions, for Romeo was not long ago in love with Rosaline, "Young men's love, then, lies not truly in their hearts, but in their eyes." (Act2 Scene3 lines 67-68) (Friar Lawrence) But as time progresses his thoughts change and he agrees to marry Romeo and Juliet, His intentions were not for the best of Romeo and Juliet but to gain prais... ...s to dealing with situations she acts much more mature, and she plays things much more safely than him. Although her character is very open she, still make sure the decisions she makes in life are the right ones. When she was about to take the potion she thought of all the different scenarios and chose which one was best for her, this is the complete opposite to Romeo. One of the key issues of the play was FATE. . One of the key issues of the play was FATE. You could say all of the events had a certain path and that one thing lead to another. Overall every single character is somewhat involved in the deaths of the star-crossed lovers, and even though they did not kill them literally, they all contributed in leading them in that direction. Works Cited Shakespeare, William. "Romeo and Juliet" 1597. Clark and Wright

Shadow Of A Doubt Essay examples -- essays research papers

Shadow of a Doubt Shadow of a Doubt is an Alfred Hitchcock film that was shot on location in the 1940's town of Santa Rosa, California. The town itself is representative of the ideal of American society. However, hidden within this picturesque community dark corruption threatens to engulf a family. The tale revolves around Uncle Charlie, a psychotic killer whose namesake niece, a teenager girl named Charlie, is emotionally thrilled by her Uncles arrival. However her opinion slowly changes as she probes into her mysterious uncle. In the film, director/producer Alfred Hitchcock blends conventions of film noir with those of a small town domestic comedy as a means of commenting on the contradictions in American values. In the beginning the film is immediately set up in the film noir style. Under the opening credits a shadowy backround image is shown kaleidoscopically. Couples dressed in elegant ballroom gowns and suits waltz together dizzyingly as the "Merry Widow Waltz" plays. The scene has nothing to do with the drama to follow (until Charlie's crimes are revealed.) The titles dissolve in to a panoramic view of a bridge, further dissolves take us first to junkyard and then to a scene of children playing in the street. The city is shown as a dirty, dark place. We are taken to a Philadelphia rooming house (shown with a number 13 on the door.) Inside we are introduced to "Uncle Charlie" (Joseph Cotten). He is reclining stiffly in bed during the day in a seedy room. . He plays with the phallic cigar that he is smoking, seemingly bitter and cynical. On the bedside table next to seemingly indifferent and fatigued man is and an open billfold with a carelessly strewn pile of bill s on top (some of the bills have fallen to the floor and lie strewn around). The overweight, middle-aged landlady knocks on the door and enters, identifying him as Mr. Spencer and informing him that two men have been asking for him. As per his instructions to not disturb him, she didn't let them in, however, they have not left, instead they retreated to the street corner to stake out the boarding house. Noticing that he looks exhausted and depressed (he passively remains on his bed during their entire conversation), she suggests that he should get some rest. Then she notices his money cluttered all about and hurries forward to straighten it ... ...of all responsibility (for, of course, there is no way that a normal person could ever kill.) In keeping with this principle the film attempts to absolve Young Charlie from all responsibility in her Uncle's death, for it is seen as an accident that occurred when Young Charlie was fighting her Uncle in self-defence. In the final stages of the film we are brought back to the small town introduced to us in the beginning, this time, however, it is in morning for a beloved son. Charlie's death has brought Graham back to Young Charlie. We can see the good side has won the battle for her. As in early situational Charlie has learned her moral lesson and the episode may end. This paper has attempted to investigate the ways in which Alfred Hitchcock blended conventions of film noir with those of a small town domestic comedy. It first looked at the opening scenes of the film in which the two conventions were introdruced. It then went on to analyse the film with the aid of Robin Wood's article Ideology, Genre, Auteur. From these two forms we can see that film noir and small town comedy were used as a means of commenting on the contradictions in American values.